ABSTRACT
Objective: To establish HPLC fingerprint chromatogram analysis for Mahuang Xuanfei Zhike syrup. Methods: The separation was performed on a Waters SunFire C18 column (250 mm × 4. 6 mm,5 μm), the mobile phase consisted of acetonitrile-0. 2% phosphoric acid with gradient elution at the flow rate of 1. 0 ml·min-1 , the detection wavelength was at 277 nm,the column temperature was at 30℃, and the sample size was 10 μl. Results: The precision, repeatability and stability of the fingerprint were measured. The fingerprints of 10 samples of Mahuang Xuanfei Zhike syrup revealed that there were twenty-four common peaks, and a-mong them, eight peaks were identified to Chinese, chlorogenic acid, caffeic acid, forsythin A, luteolin, apigenin, carotene and wild iris. Conclusion:The repeatability and information of chromatogram peaks of the method are satisfied, which provides a credible meth-od for controlling the quality of Mahuang Xuanfei Zhike syrup.